Amyloid beta (free N-term, low APP reactive) Mouse Monoclonal Antibody [Clone ID: 19H11]

CAT#: AM00007BT-N

Amyloid beta (free N-term, low APP reactive) mouse monoclonal antibody, clone 19H11, Biotin

Specifications

Product Data

• Clone Name: 19H11
• Applications: ELISA, WB
• Recommended Dilution: ELISA: Use at 0.05 µg/ml.
  Immunoblotting: 1 µg/ml for HRPO/ECL detection
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: N-terminal peptide conjugated to KLH.
  Epitope: N-Terminus of Amyloid beta-A4: NH2 - D A E F R . .
• Specificity: This antibody recognizes the free N-Terminus of the bA4 polypeptide with high preference and shows only minor crossreactions with APP.
• Formulation: 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrosee.
  Label: Biotin
  State: Liquid purified IgG fraction.
• Concentration: 0.5 mg/ml
• Purification: Subsequent Thiophilic Adsorption andSize Exclusion Chromatography
• Conjugation: Biotin
• Background: The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).