beta Catenin (CTNNB1) pTyr654 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1B11]

CAT#: AM00016BT-N

beta Catenin (CTNNB1) pTyr654 (incl. pos. control) mouse monoclonal antibody, clone 1B11, Biotin

Specifications

Product Data

• Clone Name: 1B11
• Applications: WB
• Recommended Dilution: Western Blot.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
• Specificity: This antibody specifically recognizes β-catenin phosphorylated at tyrosine 654 at 90 kDa and gamma-catenin at 70 kDa. Due to sequence homologies of the phospho-epitope surrounding pY 654 there may be crossreactions with other proteins (e.g. Annexin A11 at 56 kDa, gamma-catenin at 70 kDa, Desmoplakin at 81 kDa, TNFalpha-induced protein 2 at 73 kDa, Ribosomal protein S6 kinase alpha 6 at 90 kDa).
• Formulation: PBS/0.09% Na-Azide/PEG and Sucrose
  Label: Biotin
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Size exclusion chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1499 Human
• Background: The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. Additionally, β-catenin is a substrate of both receptor and non-receptor tyrosine kinases. Tyrosine 86 and tyrosine 654 are substrates of EGF receptor and src family kinases while tyrosine 142 is a substrate of fer tyrosine kinases.
• Synonyms: CTNNB1, CTNNB, Beta-catenin