GSK3 beta (GSK3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11B9]

CAT#: AM00066BT-N

GSK3 beta (GSK3B) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 11B9, Biotin

Specifications

Product Data

• Clone Name: 11B9
• Applications: ELISA, WB
• Recommended Dilution: ELISA: Use at 0.05 µg/ml.
  Western Blot (Immunoblotting): 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Provided Postive Control: Cell lysate from untreated HepG2 cells (See Protocols for more details).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to KLH.
  Epitope: N-terminal region of GSK3beta
• Specificity: This antibody specifically recognizes GSK3beta at 46 kDa and does not crossreact with GSK3alpha.
• Formulation: PBS containing 0.09% Sodium Azide, PEG and Sucrose.
  Label: Biotin
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C.
  Thawed aliquots may be stored at 2-8°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 46 kDa
• Database Link:
  Entrez Gene 2932 Human
• Background: Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.
• Synonyms: GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B
• Note: Protocol: Positive Control: Cell lysate from untreated HepG2 cells
  
  Format: Lyophilized cell lysate from serum starved HepG2 cells.
  
  Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
  
  Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing