IGF1 Receptor (IGF1R) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7G11]

CAT#: AM00072BT-N

IGF1 Receptor (IGF1R) (C-term) (incl. pos. control) mouse monoclonal antibody, clone 7G11, Biotin

Specifications

Product Data

• Clone Name: 7G11
• Applications: WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Icluded Positive Control: Cell lysate from untreated A431 cells (See Protocols for more details).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH.
• Specificity: This antibody specifically recognizes the C-terminus of IGF1 Receptor.
• Formulation: PBS
  Label: Biotin
  State: Liquid purified IgG fraction.
  Stabilizer: PEG and Sucrose
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 97 kDa
• Database Link:
  Entrez Gene 3480 Human
• Background: The IGF1 receptor (IGF1R) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The IGF1 receptor is activated upon binding of the peptide hormones IGF1 and 2, leading to autophosphorylation of tyrosine residue 1131, 1135, and 1136 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residue 950 and 1316 regulate the assembly of signal transduction complexes.
• Synonyms: IGF-I receptor, IGF1 Receptor
• Note: Protocol: Positive Control Cell Lysate A431 Untreated
  
  Formulation: Lyophilized cell lysate from serum starved A431 cells.
  
  Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  
  Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.