HSF1 Rat Monoclonal Antibody [Clone ID: 4B4]

CAT#: AM00238PU-N

HSF1 rat monoclonal antibody, clone 4B4, Purified

Specifications

Product Data

• Clone Name: 4B4
• Applications: IF, IP, WB
• Recommended Dilution: Western blot: 1µg/ml was sufficient for ECL immunoblot in 20µg of HeLa lysate.
  Immunofluorescence.
  Immunoprecipitation.
• Reactivities: Human, Mouse, Rat
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant mouse HSF1 protein; epitope mapping to amino acids 378-395
• Specificity: Highly specific for HSF1. Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.
• Formulation: PBS pH 7.4, 50% Glycerol, 0.09% Sodium azide
  State: Purified
  State: Liquid
• Concentration: lot specific
• Purification: Immunoglobulin fraction purified on Protein G
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3297 Human
• Background: HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents; binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA.
• Synonyms: HSTF1, HSF-1