TGF beta 1 (TGFB1) Mouse Monoclonal Antibody

CAT#: AM01369PU-N

TGF beta 1 (TGFB1) mouse monoclonal antibody, Azide Free

Product Images

Formalin-Fixed Paraffin-Embedded Sections of Human pancreas adenocarcinoma tissue stained with TGFB1 Antibody Cat.-No AM01369PU-N at 5.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

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Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant Human TGFB1 when used in conjunction with compatible secondary reagents.
  Western Blot: To detect Human TGFB1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGFB1 is 2.0-4.0 ng/lane, under non-reducing conditions.
  In a Dot Blot, a 1/500 dilution of this antibody exhibited complete cross reactivity with Human TGFB2 and TGFB3 isotypes. Reduced cross reactivity was shown at a 1/1000 dilution.
  Immunohistochemistry on Paraffin Sections: 5.0 μg/ml with an overnight incubation at 4°C.
  An HRP-labeled polymer detection system was used with an Alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Highly pure (> 98%) Insect cells derived recombinant Human TGFB1 (insect derived)
• Specificity: This antibody detects Human TGF-β1.
  Other species not tested.
• Formulation: PBS without preservatives
  State: Azide Free
  State: Lyophilized (sterile filtered) purified IgG fraction from Tissue Culture Supernatant
• Reconstitution Method: Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7040 Human
• Synonyms: TGFB, Transforming growth factor beta-1, TGF-beta-1