Cd80 Rat Monoclonal Antibody [Clone ID: RM80]

CAT#: AM05570PU-N

Cd80 rat monoclonal antibody, clone RM80, Purified

Specifications

Product Data

• Clone Name: RM80
• Applications: FC, IHC
• Recommended Dilution: Flow cytometry: 1/10 - 1/25, use 10 µl of the suggested working dilution to label 1x10e6 cells in 100 µl.
  Immunohistochemistry on frozen sections.
  Functional assays.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: BCL1 cells expressing CD80
• Specificity: This antibody recognises CD80 (B7-1), a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells. Expression may be significantly increased upon B lymphocytes by LPS and by IL-4.
• Formulation: Phosphate buffered saline, pH 7.4 containing 0.09% sodium azide.
  State: Purified
  State: Liquid Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein G
• Database Link:
  Entrez Gene 12519 Mouse
• Background: CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
• Synonyms: CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1