SIGLEC1 Mouse Monoclonal Antibody [Clone ID: 3B1/11]

CAT#: AM05889PU-S

SIGLEC1 mouse monoclonal antibody, clone 3B1/11, Purified

Specifications

Product Data

• Clone Name: 3B1/11
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to labell 1x105 cells in 100 µl.
  Immunohistochemistry on Frozen Sections.
  Positive Control: Porcine spleen.
  Western Blot: Detects a band of approximately 190kD in alveolar macrophage extracts under non-reducing conditions.
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Porcine alveolar macrophages.
• Specificity: This antibody is specific for the Porcine CD169 cell surface antigen, also known as Sialoadhesin, which is expressed by subsets of cells of the macrophage/monocyte cell lineage. CD169 is preferentially expressed on cells in the late stages of maturation. It is expressed strongly on alveolar and tissue macrophages, but weakly on blood monocytes.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 397623 Pig
• Background:

  Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.

• Synonyms: Sialoadhesin, Siglec-1