Siglec1 Rat Monoclonal Antibody [Clone ID: 3D6.112]

CAT#: AM05902LE-N

Siglec1 rat monoclonal antibody, clone 3D6.112, Low Endotoxin

Specifications

Product Data

• Clone Name: 3D6.112
• Applications: FC, IF, IHC
• Recommended Dilution:

  Immunocytochemistry.
  Flow Cytometry: 1/100-1/1000.
  Immunohistochemistry on Frozen Sections: 1/50-1/100. We recommend using fixation with either 2% paraformaldehyde or ethanol for optimal results.

• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Sialoadhesin. Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
• Specificity: This antibody recognises Mouse CD169, a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
• Formulation: PBS
  State: Low Endotoxin
  State: Liquid purified IgG fraction
  Stabilizer: None
  Preservative: None
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 20612 Mouse
• Background: Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
• Synonyms: Sialoadhesin, Siglec-1