CD11b Rat Monoclonal Antibody [Clone ID: 3A33]

CAT#: AM08031PU-N

CD11b rat monoclonal antibody, clone 3A33, Purified

Product Images

Immunofluoscent Staining: BALB/c splenocytes were incubated with Rat anti-Mouse CD11b and then stained with Goat anti-Rat IgG (H+L)-FITC. Large cells were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 g/10e6 cells.

Specifications

Product Data

• Clone Name: 3A33
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
  Immunohistochemistry on Frozen Sections. (Ref.13)
  Immunoprecipitation. (Ref.1)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Peritoneal macrophages from C57 B1/6 x DBA/2 F1 hybrid mice. (Ref.1)
• Specificity: This antibody is specific to Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer.
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Background: CD11b (Integrin alpha M subunit) combines with CD18 (Integrin beta2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). (Ref.2-6) Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. (Ref.2,7-10) In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process. (Ref.4,11) Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. (Ref.12)
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1