Cd40 Rat Monoclonal Antibody [Clone ID: 1C10]

CAT#: AM08042PU-N

Cd40 rat monoclonal antibody, clone 1C10, Purified

Specifications

Product Data

• Clone Name: 1C10
• Applications: FC, FN, IP
• Recommended Dilution: Flow Cytometry. (Ref.2)
  Immunoprecipitation. (Ref.2)
  Functional Assay: In vitro induction of proliferation of mature B lymphocytes. (Ref.2)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody is specific to Mouse CD40 (Mr. 45-50 kDa)
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 0.5 mg/ml
• Database Link:
  Entrez Gene 21939 Mouse
• Background: CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs. (Ref.1-5) CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow, with the levels of CD40 expression increasing thereafter during B-cell maturation. Immature B cells (IgM+IgDloB220lo) express intermediate levels of CD40, whereas mature B cells (IgM+IgDhiB220hi) express high levels. (Ref.4) CD40 has a central role in B cell growth and differentiation, and signalling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE. (Ref.6)
  The agonistic 1C10 antibody closely resembles gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors. (Ref.2)
• Synonyms: TNFRSF5, CDw40, Bp50