CD11a / ITGAL Rat Monoclonal Antibody [Clone ID: M17/4]

CAT#: AM12064FC-N

CD11a / ITGAL rat monoclonal antibody, clone M17/4, FITC

Product Images

Surface staining of murine monocytes using anti-CD11a monoclonal antibody (cloneM17/4) FITC.

Specifications

Product Data

• Clone Name: M17/4
• Applications: FC
• Recommended Dilution: Flow Cytometry: 3 µg/ml.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
• Specificity: The rat monoclonal antibody M17/4 reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
• Concentration: 0.5 mg/ml
• Conjugation: FITC
• Background: CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions.
• Synonyms: Integrin alpha-L, LFA1, LFA-1