CD45 / LCA (CD45RC) Rat Monoclonal Antibody [Clone ID: IBL-8]

CAT#: AM31873FC-L

CD45 / LCA (CD45RC) rat monoclonal antibody, clone IBL-8, FITC


USD 445.00

2 Weeks*

Size
    • 300 ug

Product Images

Specifications

Product Data
Clone Name IBL-8
Applications FC
Recommended Dilution Immunohistochemistry on acetone-fixed frozen sections.
Flow Cytometry.
Immunoprecipitation.
Reactivities Mouse
Host Rat
Isotype IgG1
Clonality Monoclonal
Immunogen Mouse Spleen Cells
Donor: Wistar spleen
Fusion Partner: Sp-2/0 Ag14
Formulation PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Label: FITC
State: Liquid purified Ig fraction
Label: Fluorescein isothiocyanate isomer 1
Concentration 0,1 mg/ml
Conjugation FITC
Background CD45 (L-CA) is a transmembrane phosphotyrosine phosphatase expressed on leukocytes. Anti-mouse CD45RC is against the exon C-dependent RC isoform and reacts strongly with B cells, and less intensely with most CD8+ T cells. It does not recognize CD4+ T cells. Also, myeloid cells do not express the RC isoform.
Synonyms PTPRC, Leukocyte common antigen, L-CA, T200
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 0.5 μg of this antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 μl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.5 μg/10e6 cells
Isotypic Control: FITC Rat IgG1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.