CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: BEAR-1]

CAT#: AM32402PU-N

CD11b (ITGAM) mouse monoclonal antibody, clone BEAR-1, Purified

Specifications

Product Data

• Clone Name: BEAR-1
• Applications: IF, IHC, IP
• Recommended Dilution: Fluorescence Microscopy or Flow Cytometry (2 µg / 5x10⁵ cells/test).
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Typical Starting Dilution: 1/10.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody clone Bear-1 reacts with the CD11b alpha chain of the CD11b/CD18 (p165, 95) integrin heterodimer complex present on Human NK cells, monocytes, and granulocytes.
  The recognized structure is the alpha chain of a heterodimer glycoprotein of Mw 165kD (alpha chain) and 95kD (beta chain).
• Formulation: PBS
  State: Purified
  State: Liquid (0.2 µm filtered) purified IgG fraction
  Stabilizer: 0.1% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha M
• Database Link:
  Entrez Gene 3684 Human
• Background: CD11b is an alpha chain integrin that is expressed as heterodimer noncovalently associated with CD18. CD11b is also designated as CR3, Mac-1, Mo-1 in integrin alphaM subunit and has a molecular weight of 165-170 kDa. The protein mediates PMN and monocytes adherence to endothelium and subsequently PMN extravasation to sites of inflammation. CD11b/CD18 interacts with both matrix and cell surface proteins including the complement component iC3b, extracellular matrix proteins and the third extracellular domain of CD54. CD11b is expressed on human NK-cells, monocytes, granulocytes, T-cells, B-cells and dendritic cells. After activation the expression is upregulated.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1