PDZK1 Mouse Monoclonal Antibody [Clone ID: AT1A2]

CAT#: AM50029PU-N

PDZK1 mouse monoclonal antibody, clone AT1A2, Purified

Product Images

The cell lysates of HeLa (40ug) and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDZK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT1A2
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
  Recommended starting dilution is 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human PDZK1 (1-519aa) purified from E. coli
• Specificity: This antibody recognizes Human PDZK1.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens PDZ domain containing 1 (PDZK1), transcript variant 2
• Database Link:
  Entrez Gene 5174 Human
• Background: PDZK1 is PDZ domain-containing scaffolding protein with a 519 amino acid protein. PDZK1 mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. This protein is expressed in the kidney, pancreas, liver, gastrointestinal tract, and adrenal cortex. In situ hybridization experiments showed that the expression of PDZK1 was limited to epithelial cells. In addition, PDZK1 was overexpressed in selected tumors of epithelial origin.
• Synonyms: PDZK1, NHERF3, NHERF-3, NHE-RF3, NaPi-Cap1, CAP70