Timd4 Rat Monoclonal Antibody [Clone ID: RMT4-54]

CAT#: AM60019PU-S

Timd4 rat monoclonal antibody, clone RMT4-54, Purified

Product Images

Mouse TIM-4 transfected NRK cells stained with rat anti mouse TIMD4 / TIM-4 antibody.

Specifications

Product Data

• Clone Name: RMT4-54
• Applications: FC, IF, IHC
• Recommended Dilution: Flow cytometry: 1/25-1/200, use 10µl of this suggested working dilution to label 10e6 cells in 100µl.
  Immunofluorescence.
  Immunohistochemistry on Paraffin sections.
  Positive control: Mouse spleen.
  For functional assays Cat.-No. AM60019LE-N is recommended.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse TIMD4 (amino acids 1-288), coupled with the Fc portion of mouse IgG2a
• Specificity: Clone RMT4-54 specifically recognises mouse Tim-4.
  Studies have shown that clone RMT4-54 significantly inhibits the efficient phagocytosis of apoptotic cells by PRMs (peritoneal resident Mac1⁺ cells), but not PEMs (peritoneal exudate Mac1⁺ cells) [Nakayama, 2009].
• Formulation: PBS containing 0.09% Sodium azide
  State: Purified
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein G
• Gene Name: T cell immunoglobulin and mucin domain containing 4
• Database Link:
  Entrez Gene 276891 Mouse
• Background: TIMD4 / TIM-4 (T-cell immunoglobulin mucin 4), a transmembrane glycoprotein and member of the immunoglobulin superfamily, exclusively expressed by antigen-presenting cells, which acts as the natural ligand of TIM-1, involved in T-cell expansion and cytokine production.
  TIMD4 is reported to be crucial for the phagocytosis of apoptotic cells by peritoneal macrophages, and studies have shown that interaction between TIM-1 on Th2 cells and TIM-4 on DCs, enhances Th2 cell function. The TIMD4 gene locus is associated with predisposition to asthma in both mice and humans and may be considered as a candidate disease gene for asthma.
• Synonyms: TIMD-4, TIM4, TIM-4, T-cell membrane protein 4