Eotaxin 3 (CCL26) Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommended Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (100 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required. ELISA: Indirect: To detect hEotaxin-3 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3. Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. In conjunction with Biotinylated Anti-Human Eotaxin-3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98%) E.coli derived, 8.4 kDa recombinant human Eotaxin-3 |
Specificity | This antibody detects Eotaxin-3. |
Formulation | PBS, pH 7.2 State: Aff - Purified State: Sterile filtered lyophilized Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml. |
Purification | Immunoaffinity chromatography |
Database Link | |
Background | Eotaxin 3 is a recently discovered CC chemokine. It binds to the CCR3 receptor and is highly chemotactic for eosinophils and basophils. |
Synonyms | C-C motif chemokine 26, Small-inducible cytokine A26, SCYA26, MIP4 alpha, IMAC, TSC-1, TSC1 |
Reference Data |
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