IL1RA (IL1RN) Rabbit Polyclonal Antibody

CAT#: AP05027BT-N

IL1RA (IL1RN) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: ELISA: 2 µg/ml in a two-site ELISA with unconjugated IL-1ra antibody. Typically the ELISA has a working range of 0 - 20000 pg/ml and a sensitivity of <50 pg/ml with the appropriate standard. Recommended sample volumes are 25 - 200 µl. Recovery of hIL-1RA in human plasma is typically > 85% in the ELISA.
  Immunoprecipitation: using a double-antibody method.
  Western blot: 10 µg/ml.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human recombinant interleukin 1 receptor antagonist (hIL-1RA).
• Specificity: This antibody recognizes IL-1RA. Does not recognize hIL-1a, hIL-1b or a panel of other cytokines.
• Formulation: Phosphate buffered saline with 0.08% sodium azide
  Label: Biotin
  State: Liquid purified Ig (0.2 µm sterile filtered)
• Concentration: lot specific
• Purification: > 95 % by SDS PAGE
• Conjugation: Biotin
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 1 receptor antagonist
• Database Link:
  Entrez Gene 3557 Human
• Background: IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Synonyms: IL1F3, IL-1ra, IL-1RN, IRAP, IL1 inhibitor, ICIL-1RA