AIRE (C-term) Goat Polyclonal Antibody

CAT#: AP08252PU-N

AIRE (C-term) goat polyclonal antibody, Aff - Purified

Product Images

Thymus: Formalin-Fixed Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, R
• Recommended Dilution: ELISA: 1/32000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  RIA: 0.01 - 0.03 µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to the C-Terminal of Human AIRE (NP_000374.1, NP_000649.1).
  Epitope: aa534-545
• Specificity: This antibody recognize two of the tree reported isoforms (NP_000374.1 and NP_000649.1) from the C-terminus of human AIRE.
• Formulation: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 326 Human
• Background: AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
• Synonyms: Autoimmune regulator