HES6 Rabbit Polyclonal Antibody

CAT#: AP20454PU-N

HES6 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HES-6 antibody (Cat.-No AP20454PU-N) in extracts from HeLa cells.

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  Immunohistochemistry analysis of HES-6 antibody (Cat.-No AP20454PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of HES-6 protein.
  (region surrounding Ala6)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Predicted Protein Size: ~30 kDa
• Gene Name: Homo sapiens hes family bHLH transcription factor 6 (HES6), transcript variant 1
• Database Link:
  Entrez Gene 55502 Human
• Background: The Drosophila hairy and enhancer of split genes encode basic helix-loophelix (bHLH) transcriptional repressors that function in the notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homologues of Drosophila hairy and enhancer of split are the HES gene family members, HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homologue of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N-box (CACNAG) as opposed to the E-box (CANNTG). HES2 binds to both N- and E-box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues.
• Synonyms: Transcription cofactor HES-6, C-HAIRY1, BHLHB41

Reference Data

• Protein Families: Druggable Genome, Transcription Factors