Pepsinogen II (PGC) Rabbit Polyclonal Antibody

CAT#: AP20778PU-N

Pepsinogen II (PGC) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of Pepsin C antibody (Cat.-No.: AP20778PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of Pepsin C antibody (Cat.-No.: AP20778PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Pepsin C protein.
  (region surrounding Phe306)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 42 kDa
• Gene Name: Homo sapiens progastricsin (pepsinogen C) (PGC), transcript variant 1
• Database Link:
  Entrez Gene 5225 Human
  UniProt ID P20142
• Background: Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, Pepsinogen, by removal of a 41 amino acid segment from the amino end. Pepsinogen is synthesized in the stomach lining; hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for Pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. It shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of Pepsinogen and Pepsin, especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine.
• Synonyms: Pepsinogen C, Progastricsin, Gastricsin, PGC

Reference Data

• Protein Families: Protease, Secreted Protein