ATM pSer1981 Rabbit Polyclonal Antibody

CAT#: AP20875PU-N

ATM pSer1981 rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry (IHC) analyzes of p-ATM antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human ATM around the phosphorylation site of Serine 1981.
• Specificity: This antibody detects endogenous levels of p-ATM protein only when phosphorylated at Ser1981.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 350 kDa
• Gene Name: Homo sapiens ATM serine/threonine kinase (ATM)
• Database Link:
  Entrez Gene 472 Human
• Background: The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4- kinases and the second group comprises the PIK-related kinases. The PIKrelated kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and they display delays in p53 induction. Cultured cells respond to breaks in double stranded DNA by immediate activation of ATM through autophosphorylation on Serine 1981. Most human tissues, however, contain the nonphosphorylated, inactive form of Atm.
• Synonyms: Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATDC, TEL1, TELO1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Apoptosis, Cell cycle, p53 signaling pathway