ME1 Rabbit Polyclonal Antibody

CAT#: AP21198PU-N

ME1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Malic enzyme 1 antibody in extracts from HeLa cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Malic enzyme 1 protein.
  (region surrounding Tyr500)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 64 kDa
• Gene Name: Homo sapiens malic enzyme 1, NADP(+)-dependent, cytosolic (ME1)
• Database Link:
  Entrez Gene 17436 MouseEntrez Gene 24552 RatEntrez Gene 4199 Human
• Background: ME1 (Malic enzyme 1), also known as NADP-ME, MES or HUMNDME, is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family. Expressed ubiquitously with highest expression in liver and white adipose tissue, ME1 functions as an NADP-dependent enzyme that catalyzes the conversion of S-malate and NADP to pyruvate, carbon dioxide and NADPH (a reducing agent that participates in fatty acid biosynthesis). Through its ability to catalyze the reversible oxidative decarboxylation of malate, ME1 links the citric acid and glycolytic cycles. ME1 exists as a homotetramer that uses divalent metal cations, such as magnesium or manganese, as cofactors. The expression of ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet, indicating that ME1 may play an important role as a housekeeping protein within the cell.
• Synonyms: ME1, NADP-dependent malic enzyme

Reference Data

• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism