PDK4 (C-term) Rabbit Polyclonal Antibody

CAT#: AP22786PU-N

PDK4 (C-term) rabbit polyclonal antibody, Purified

Product Images

(LEFT) The PDK4 Antibody is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate. (RIGHT) Western blot analysis of PDK4 in 293 cell lysates (2 ug/lane) either non-transfected (Lane 1) or transiently transfected with the PDK4 gene (Lane 2).

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  Human Adrenal (formalin-fixed, paraffin-embedded) stained with PDK4 antibody followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Pancreas (formalin-fixed, paraffin-embedded) stained with PDK4 antibody followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunofluorescence: 1/100.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: PDK4 antibody was raised against kLH conjugated synthetic peptide between amino acids 382-410 from the C-terminal region of human PDK4.
• Specificity: This antibody regognizes Pyruvate Dehydrogenase Kinase Isoenzyme 4 (PDK4).
• Formulation: PBS containing 0.09% sodium azide
  State: Purified
  State: Liquid purified Ig fraction
• Purification: Ammonium Sulfate Precipitation
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens pyruvate dehydrogenase kinase 4 (PDK4)
• Database Link:
  Entrez Gene 27273 MouseEntrez Gene 5166 Human
• Background: PDK4 belongs to the PDK/BCKDK protein kinase subfamily. PDK4 contributes to the regulation of glucose metabolism by inhibiting the mitochondrial pyruvate dehyrogenase complex by phosphorylating the E1 alpha subunit. PDK4 is upregulated in many tissues of the body during starvation.
• Synonyms: Pyruvate dehydrogenase kinase isoform 4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase