HIP2 (UBE2K) (144-157) Goat Polyclonal Antibody

CAT#: AP23819PU-N

HIP2 (UBE2K) (144-157) goat polyclonal antibody, Aff - Purified

Product Images

Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/2000.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
  Western Blot: 1/2000, 1 - 3 µg/ml.
• Reactivities: Bat, Bovine, Canine, Chicken, Equine, Goat, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus, Zebrafish
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C-terminus of human HIP2 (aa 144-157)
• Specificity: This antibody reacts to the C-term of UBE2K.
  It is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA as stabilizer and 0.02% sodium azide as preservative
  State: Aff - Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript variant 3
• Database Link:
  Entrez Gene 53323 MouseEntrez Gene 289623 RatEntrez Gene 3093 Human
• Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein.
• Synonyms: HIP-2, Ubiquitin-conjugating enzyme E2 K, E2(25K), E2-25K, Ubiquitin-protein ligase, LIG, Ubiquitin carrier protein, Huntingtin-interacting protein 2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis