PKM2 (PKM) (36-40) Rabbit Polyclonal Antibody

CAT#: AP26067PU-S

PKM2 (PKM) (36-40) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from HepG2 and 293 cells using PKM2 Antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:1000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa. 36~40
• Specificity: This antibody detects endogenous levels of total PKM2 protein.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity-chromatography using epitope-specific peptide
• Storage: Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 1
• Database Link:
  Entrez Gene 5315 Human
• Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
• Synonyms: PK2, PK3, PKM, CTHBP, M2-PK, THBP1, OIP3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase muscle
• Note: Molecular weight: 60 kDa

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus