Metabotropic Glutamate Receptor 5 (GRM5) Chicken Polyclonal Antibody
CAT#: AP32843PU-N
Metabotropic Glutamate Receptor 5 (GRM5) chicken polyclonal antibody, Purified
Specifications
Product Data | |
Applications | IF, IHC |
Recommended Dilution | Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at 1/2000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. |
Reactivities | Human, Mouse |
Host | Chicken |
Isotype | Ig |
Clonality | Polyclonal |
Immunogen | Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the Human (NP_001137303.1, NCBI) and Mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Formulation | PBS, pH 7.2 State: Purified State: Liquid purified IgY fraction Preservative: 0.02% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography |
Storage | Store undiluted at 2-8°C. This product is photosensitive and should be protected from light. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Synonyms | Metabotropic glutamate receptor 5, GPRC1E, MGLUR5 |
Reference Data |
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