BAHD1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP50338PU-N

BAHD1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of BAHD1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). BAHD1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  BAHD1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow cytometry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 751~780 amino acids from the C-terminal region of human BAHD1
• Specificity: This antibody reacts to BAHD1.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens bromo adjacent homology domain containing 1 (BAHD1), transcript variant 1
• Database Link:
  Entrez Gene 22893 Human
• Background: BAHD1 is a heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. It may act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. It represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins.
• Synonyms: KIAA0945
• Note: Molecular Weight: 84652 Da