DIAPH3 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51261PU-N

DIAPH3 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of DIAPH3 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). This demonstrates the DIAPH3 antibody detected the DIAPH3 protein (arrow)

Specifications

Product Data

• Applications: WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1060-1090 amino acids from the C-terminal region of human DIAPH3.
• Specificity: This antibody recognizes Human DIAPH3 (C-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens diaphanous related formin 3 (DIAPH3), transcript variant 1
• Database Link:
  Entrez Gene 81624 Human
• Background:
  DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).
• Synonyms: DIAP3, Diaphanous homolog 3, Diaphanous-3, DRF3
• Note: Molecular Weight: 136926 Da

Reference Data

• Protein Pathways: Regulation of actin cytoskeleton