EFEMP2 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51375PU-N

EFEMP2 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Fibulin-4 (arrow) in mouse NIH-3T3 cell line lysates (35ug/lane) using Fibulin-4 antibody. (C-term).

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  Western blot analysis of Fibulin-4 (arrow) in HepG2 cell line lysates (35ug/lane) using Fibulin-4 antibody. (C-term).

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  Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram) using Fibulin-4 antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 393-422aa) of human Fibulin-4
• Specificity: This antibody recognizes Fibulin-4 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens EGF containing fibulin like extracellular matrix protein 2 (EFEMP2), transcript variant 1
• Database Link:
  Entrez Gene 58859 MouseEntrez Gene 30008 Human
• Background: EFEMP2 / Fibulin-4 has been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein contains four EGF2 domains and six calcium-binding EGF2 domains. This protein is necessary for elastic fiber formation and connective tissue development.
• Synonyms: Fibulin 4, EFEMP2, FBLN4, UNQ200/PRO226, Protein UPH1
• Note: Molecular Weight: 49405 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein