GCLC (N-term) Rabbit Polyclonal Antibody

CAT#: AP51800PU-N

GCLC (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GCLC / GLCLC Antibody (N-term) in Jurkat cell line lysates (35ug/lane). This demonstrates the GCLC antibody detected the GCLC protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma reacted with GCLC / GLCLC Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining

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  Confocal immunofluorescent analysis of GCLC / GLCLC Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of Jurkat cells using GCLC / GLCLC Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of Human GCLC / GLCLC
• Specificity: This antibody recognizes Human GCL / GLCLC (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 2
• Database Link:
  Entrez Gene 2729 Human
• Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
• Synonyms: GCS heavy chain, GLCL, GLCLC, Gamma-ECS
• Note: Molecular Weight: 72766 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways