IDH3G (C-term) Rabbit Polyclonal Antibody

CAT#: AP52147PU-N

IDH3G (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of IDH3G Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). IDH3G (arrow) was detected using the purified Pab.

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  Flow cytometric analysis of NCI-H460 cells using IDH3G Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 364~393 amino acids from the C-terminal region of human IDH3G
• Specificity: This antibody recognizes Human Isocitric dehydrogenase gamma / IDH3G (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens isocitrate dehydrogenase 3 (NAD(+)) gamma (IDH3G), transcript variant 1
• Database Link:
  Entrez Gene 3421 Human
• Background:
  Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia.
• Synonyms: H-IDHG; IDH-gamma
• Note: Molecular Weight: 42794 Da

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways