NEDD4 (C-term) Rabbit Polyclonal Antibody

CAT#: AP52847PU-N

NEDD4 (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of NEDD4 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue reacted with NEDD4 Antibody (C-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for Clinical relevance has not been evaluated.

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  Flow cytometric analysis of NCI-H460 cells using NEDD4 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 977~1007 amino acids from the C-terminal region of human NEDD4
• Specificity: This antibody recognizes Human and Mouse NEDD4 (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 1
• Database Link:
  Entrez Gene 17999 MouseEntrez Gene 4734 Human
• Background: Nedd4 is one of a group of mouse genes that show developmentally regulated expression in mouse embryonic brain. Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.
• Synonyms: KIAA0093
• Note: Molecular Weight: 149059 Da (Isoform 4.;104162 Da) Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis