PCDHA9 (N-term) Rabbit Polyclonal Antibody

CAT#: AP53203PU-N

PCDHA9 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in NCI-H460, K562 cell line lysates (35ug/lane) using PCDHA9 antibody. (N-term). This demonstrates the PCDHA9 antibody detected the PCDHA9 protein (arrow).

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  Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram) using PCDHA9 antibody. (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: This antibody is suitable for flow cytometry and Western blotting.
  The suggested dilution is:
  Flow cytometry: 1/10-1/50.
  Western blotting: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 233-26aa) of human PCDHA9.
• Specificity: This antibody detects human PCDHA9 at N-term.
• Formulation: PBS with 0.09% (W/V) Sodium Azide.
  State: Aff - Purified
• Concentration: lot specific
• Purification: Purified through a protein A column; followed by peptide affinity purification.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens protocadherin alpha 9 (PCDHA9), transcript variant 2
• Database Link:
  Entrez Gene 9752 Human
• Background: The PCDHA9 gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.
• Synonyms: Protocadherin alpha-9, PCDH-alpha-9, KIAA0345
• Note: Molecular Weight: 91;297 Da Isoform2. 102402 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane