PKM2 (PKM) (N-term) Rabbit Polyclonal Antibody

CAT#: AP53318PU-N

PKM2 (PKM) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of PKM2 (arrow) using PKM2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).

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  Formalin fixed, paraffin embedded human lung carcinoma stained with KM2 Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of 293 cells using KM2 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 123~153 amino acids from the N-terminal region of Human PKM2.
• Specificity: This antibody recognizes Human PKM2 (N-term).
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens pyruvate kinase, muscle (PKM), transcript variant 4
• Database Link:
  Entrez Gene 5315 Human
• Background:
  PKM2 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
• Synonyms: PK2, PK3, PKM, CTHBP, M2-PK, THBP1, OIP3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase muscle
• Note: Molecular Weight: 57937 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus