PREX1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP53441PU-N

PREX1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

PREX1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PREX1 antibody detected the PREX1 protein (arrow).

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  PREX1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PREX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  PREX1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/10 - 1/50.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1133-1163 amino acids from the C-terminal region of human PREX1
• Specificity: This antibody reacts to PREX1.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 186203 Da
• Gene Name: Homo sapiens phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 (PREX1)
• Database Link:
  Entrez Gene 57580 Human
• Background: The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.
• Synonyms: P-Rex1, KIAA1415

Reference Data

• Protein Pathways: Chemokine signaling pathway