PRUNE (PRUNE1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP53474PU-N

PRUNE (PRUNE1) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PRUNE (arrow) in Jurkat cell line lysates (35ug/lane) using PRUNE antibody. (C-term).

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  Immunohistochemistry analysis in prostate carcinoma (Formalin-fixed, Paraffin-embedded) using PRUNE antibody. (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRUNE Antibody (C-term) for IHC; Clinical relevance has not been evaluated.

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  Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) using PRUNE antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 367-397aa) of human PRUNE
• Specificity: This antibody recognizes PRUNE at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens prune exopolyphosphatase (PRUNE), transcript variant 1
• Database Link:
  Entrez Gene 58497 Human
• Synonyms: Protein prune homolog, hPrune, HTcD37, DRES17, DRES-17
• Note: Molecular Weight: 50200 Da

Reference Data

• Protein Pathways: Purine metabolism