RHEB (C-term) Rabbit Polyclonal Antibody

CAT#: AP53656PU-N

RHEB (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of RHEB Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the RHEB antibody detected the RHEB protein (arrow).

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  Formalin fixed, paraffin embedded human skeletal muscle stained with RHEB Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RHEB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Western Blot: 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Bovine, Human, Mouse, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 104-134 amino acids from the C-terminal region of Human RHEB.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens Ras homolog enriched in brain (RHEB)
• Database Link:
  Entrez Gene 19744 MouseEntrez Gene 26954 RatEntrez Gene 6009 Human
• Background: This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22
• Synonyms: RHEB2
• Note: Molecular Weight: 20497 Da

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway