Follicular Dendritic Cells Mouse Monoclonal Antibody [Clone ID: Ki-M9R]

CAT#: BM4006

Follicular Dendritic Cells mouse monoclonal antibody, clone Ki-M9R, Purified

Product Images

Rat Spleen Frozen Section stained withBM4006 Clone Ki-M9R

• 

  During the course of an infection the number of antigen trapping cells decreases while the number of antigen presenting cells increases. Unpublished data raised in The Institute of Pathology, Michaelisstrasse 11, 2300 Kiel, Germany

Specifications

Product Data

• Clone Name: Ki-M9R
• Applications: Assay, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 5 µg/ml (1/200)
  Immunohistochemistry on Paraffin Sections: 50 µg/ml (1/20). Proteinase K pretreatment for antigen retrieval is recommended.
  Suggested Positive Control: Rat spleen.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat peritoneal macrophages.
• Specificity: This antibody reacts with subpopulation of macrophages.
  Ki-M9R detects an epitope of a 49kD cytoplasmic antigen.
  Antigen Distribution
  Tissue Sections: Ki-M9R specifically recognises antigen trapping macrophages of the B-cell system such as sinus lining cells and dendritic reticulum cells. It is also positive on splenic metallophils. Macrophages in T-cell areas are negative with Ki-M9R.
  None of the macrophages in non-lymphoid organs are detected by Ki-M 9R.
  No cross-reactivity was found with other cell types at different tissue sites except with hepatic sinusoids. In vivo adminstration of Ki-M9R reveals a direct cytotoxicity to, and the elimation of Ki-M9R positive cells. Animals survive this treatment.
  Isolated cells: Ki-M9R is negative with all peripheral blood cells and cells of the bone marrow or peritoneal cavity.
• Formulation: PBS buffer with 0.01% Thimerosal as preservative and 10 mg/ml BSA as stabilizer
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.5 ml distilled water (stock solution).
• Concentration: 1.0 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Background: Follicular dendritic cells are cells that facilitate antigen recognition by B cells in follicles. This kind of dendritic cell is not bone marrow derived and is not a kind of blood cell. It is purely a resident of the follicles of secondary lymphoid organs. B cells form germinal centers around follicular dendritic cells in lymphoid organs. Dendritic cells form from monocytes, white blood cells which circulate in the body and, depending on the right signal, can turn into dendritic cells or macrophages. The monocytes in turn are formed from stem cells in the bone marrow.
• Note: Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum
  Protocol with formalin-fixed, paraffin-embedded sections:
  The whole procedure is performed at room temperature
  1. Deparaffinize and rehydrate tissue section
  2. Incubate the tissue section with proteinase K for 7min.
  3. Wash in distilled water
  4. Block endogenous peroxidase
  5. Wash in PBS
  6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber
  10. Wash in PBS
  11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  12. Wash in PBS
  13. Counterstain with Mayer's hemalum