Macrophages Mouse Monoclonal Antibody [Clone ID: PM-2K]
Specifications
Product Data | |
Clone Name | PM-2K |
Applications | Assay, IHC |
Recommended Dilution | Immunohistochemistry on frozen section: 0.2 mg/ml (1:1000). Suggested positive control: Human tonsil. Does not react on routinely processed paraffin sections. |
Reactivities | Bovine, Canine, Feline, Human, Monkey, Porcine |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Cultured human peritoneal macrophages |
Specificity | This antibody detects Macrophages. Antigen distribution: Isolated cells: Positive on >90% of alveolar macrophages, and on 10% of adherent peritoneal cells after 1 day culture. Negative on dendritic cells, on freshly isolated blood monocytes or peritoneal cells; and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes. Tissue sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages an d macrophages in the interstitial tissues of the kidney, pancreas and many other organs. Proliferating macrophages are positive i n Gaucher's disease , multicentric reticulohistiocytosis and malignant histiocytosis. In MFH infiltrating macrophages are staine d but not tumour cells. Some bone stromal cells but not osteoclast - like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are nega tive. |
Formulation | Phosphate buffered saline pH 7.2 (PBS) State: Purified State: Lyophilized affinity purified Ig fraction Stabilizer: 5 mg/ml bovine serum albumin (BSA) Preservative: 0.05 % (v/v) Kathon CG |
Reconstitution Method | Reconstitute by adding 0.5 ml distilled water. |
Purification | |
Background | Monoclonal antibody PM-2K, together with X-4 and X-14, forms a particular group of macrophage specific antibodies which were tested at the Vth Leukocyte Typing Workshop. The PM-2K antigen was initially suspected to function as a scavenger receptor. However, experiments with CHO cells expressing Macrophage Scavenger Receptor (MSR) AI or AII showed no reactivity with this antibody. |
Synonyms | Macrophage marker |
Reference Data |
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