Cardiac Troponin I (TNNI3) Mouse Monoclonal Antibody [Clone ID: OTI4E5]

CAT#: CF500057

Carrier-free (BSA/glycerol-free) TNNI3 mouse monoclonal antibody, clone OTI4E5 (formerly 4E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (RC214740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3. Positive lysates LY424766 (100ug) and LC424766 (20ug) can be purchased separately from OriGene.

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  Anti-TNNI3 mouse monoclonal antibody (TA500057) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TNNI3 (RC214740).

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  Immunofluorescent staining of HeLa cells using anti-TNNI3 mouse monoclonal antibody (TA500057).

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  Immunofluorescent staining of A549 cells using anti-TNNI3 mouse monoclonal antibody (TA500057).

Specifications

Product Data

• Clone Name: OTI4E5
• Applications: IF, WB
• Recommended Dilution: WB 1:2500~5000, IF 1:50~100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TNNI3 (NP_000354) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.8 kDa
• Gene Name: Homo sapiens troponin I3, cardiac type (TNNI3), mRNA.
• Database Link:
  NP_000354
  Entrez Gene 21954 MouseEntrez Gene 29248 RatEntrez Gene 403566 DogEntrez Gene 698470 MonkeyEntrez Gene 7137 Human
  UniProt ID P19429
• Background: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
• Synonyms: CMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)