Pancreatic alpha amylase (AMY2A) Mouse Monoclonal Antibody [Clone ID: OTI6D4]

CAT#: CF500146

Carrier-free (BSA/glycerol-free) AMY2A mouse monoclonal antibody, clone OTI6D4 (formerly 6D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMY2A (RC202805, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMY2A. Positive lysates LY424559 (100ug) and LC424559 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from a mouse cell line by using anti-AMY2A monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded pancreas within the normal limits using anti-AMY2A (TA500146) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Specifications

Product Data

• Clone Name: OTI6D4
• Applications: IHC, WB
• Recommended Dilution: WB: 1:500, IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 300-511 of human AMY2A
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.7 kDa
• Gene Name: Homo sapiens amylase alpha 2A (AMY2A), mRNA.
• Database Link:
  NP_000690
  Entrez Gene 279 Human
  UniProt ID P04746
• Background: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides andpolysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Thehuman genome has a cluster of several amylase genes that are expressed at high levels in eithersalivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas
• Synonyms: AMY2; PA

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism