Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI8A4]

CAT#: CF500402

Carrier-free (BSA/glycerol-free) SILV mouse monoclonal antibody, clone OTI8A4 (formerly 8A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SILV (RC200663, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SILV. Positive lysates LY402062 (100ug) and LC402062 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SILV monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SILV mouse monoclonal antibody. (TA500402)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SILV mouse monoclonal antibody. (TA500402)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SILV mouse monoclonal antibody. (TA500402)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SILV mouse monoclonal antibody. (TA500402)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SILV mouse monoclonal antibody. (TA500402)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SILV mouse monoclonal antibody. (TA500402)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SILV mouse monoclonal antibody. (TA500402)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SILV mouse monoclonal antibody. (TA500402)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SILV mouse monoclonal antibody. (TA500402)

• Anti-SILV mouse monoclonal antibody (TA500402) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SILV (RC200663).

• Immunofluorescent staining of A549 cells using anti-SILV mouse monoclonal antibody (TA500402).

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Specifications

Product Data

• Clone Name: OTI8A4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC: 1:150
• Reactivities: Human, Monkey, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SILV expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.3 kDa
• Gene Name: Homo sapiens premelanosome protein (PMEL), transcript variant 3, mRNA.
• Database Link:
  NP_008859
  Entrez Gene 362818 RatEntrez Gene 481102 DogEntrez Gene 710921 MonkeyEntrez Gene 6490 Human
  UniProt ID P40967
• Background: Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity
• Synonyms: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV

Reference Data

• Protein Families: Secreted Protein, Transmembrane