SCY1 like 3 (SCYL3) Mouse Monoclonal Antibody [Clone ID: OTI7E7]

CAT#: CF500489

Carrier-free (BSA/glycerol-free) SCYL3 mouse monoclonal antibody, clone OTI7E7 (formerly 7E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3. Positive lysates LY412474 (100ug) and LC412474 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7E7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SCYL3 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.8 kDa
• Gene Name: Homo sapiens SCY1 like pseudokinase 3 (SCYL3), transcript variant 1, mRNA.
• Database Link:
  NP_065156
  Entrez Gene 57147 Human
  UniProt ID Q8IZE3
• Background: May play a role in regulating cell adhesion/migration complexes in migrating cells
• Synonyms: PACE-1; PACE1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase