MRI1 Mouse Monoclonal Antibody [Clone ID: OTI9H7]

CAT#: CF500491

Carrier-free (BSA/glycerol-free) MRI1 mouse monoclonal antibody, clone OTI9H7 (formerly 9H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRI1 (RC200993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRI1. Positive lysates LY422176 (100ug) and LC422176 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500491)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500491)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500491)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MRI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500491)

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  Anti-MRI1 mouse monoclonal antibody (TA500491) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRI1 (RC200993).

• HEK293T cells transfected with either RC200993 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRI1 antibody (TA500491), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI9H7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MRI1 (NP_115661) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.1 kDa
• Gene Name: Homo sapiens methylthioribose-1-phosphate isomerase 1 (MRI1), transcript variant 1, mRNA.
• Database Link:
  NP_001026897
  Entrez Gene 84245 Human
  UniProt ID Q9BV20
• Background: Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P) (By similarity.
• Synonyms: MRDI; MTNA; Ypr118w