TRIB2 Mouse Monoclonal Antibody [Clone ID: OTI8D11]

CAT#: CF500510

Carrier-free (BSA/glycerol-free) TRIB2 mouse monoclonal antibody, clone OTI8D11 (formerly 8D11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB2 (RC201210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB2. Positive lysates LY402872 (100ug) and LC402872 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIB2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500510)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500510)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500510)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500510)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500510)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500510)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRIB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500510)

• Immunofluorescent staining of COS7 cells using anti-TRIB2 mouse monoclonal antibody (TA500510).

• HEK293T cells transfected with either RC201210 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIB2 antibody (TA500510), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI8D11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:50, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TRIB2 (NP_067675) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.8 kDa
• Gene Name: Homo sapiens tribbles pseudokinase 2 (TRIB2), transcript variant 1, mRNA.
• Database Link:
  NP_067675
  Entrez Gene 217410 MouseEntrez Gene 28951 Human
  UniProt ID Q92519
• Background: This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene.
• Synonyms: C5FW; GS3955; TRB2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase