Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI4A1]

CAT#: CF500548

Carrier-free (BSA/glycerol-free) HSPA9 mouse monoclonal antibody, clone OTI4A1 (formerly 4A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (RC201397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9. Positive lysates LY401334 (100ug) and LC401334 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody.

• 

  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HSPA9 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

• 

  Anti-HSPA9 mouse monoclonal antibody (TA500548) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 (RC201397).

• 

  Immunofluorescent staining of HT29 cells using anti-HSPA9 mouse monoclonal antibody (TA500548).

Show all 5 images

Specifications

Product Data

• Clone Name: OTI4A1
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human HSPA9 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.7 kDa
• Gene Name: Homo sapiens heat shock protein family A (Hsp70) member 9 (HSPA9), mRNA.
• Database Link:
  NP_004125
  Entrez Gene 15526 MouseEntrez Gene 291671 RatEntrez Gene 474697 DogEntrez Gene 714506 MonkeyEntrez Gene 3313 Human
  UniProt ID P38646
• Background: The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone.
• Synonyms: CSA; GRP-75; GRP75; HEL-S-124m; HSPA9B; MOT; MOT2; MTHSP75; PBP74

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation