SHC (SHC1) Mouse Monoclonal Antibody [Clone ID: OTI1H8]

CAT#: CF501075

Carrier-free (BSA/glycerol-free) SHC1 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (RC204362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1. Positive lysates LY401060 (100ug) and LC401060 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501075, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501075, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501075, Dilution 1:50)

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  Anti-SHC1 mouse monoclonal antibody (TA501075) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1 (RC204362).

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Specifications

Product Data

• Clone Name: OTI1H8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.5 kDa
• Gene Name: Homo sapiens SHC adaptor protein 1 (SHC1), transcript variant 2, mRNA.
• Database Link:
  NP_003020
  Entrez Gene 20416 MouseEntrez Gene 85385 RatEntrez Gene 6464 Human
  UniProt ID P29353
• Background: Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span
• Synonyms: SHC; SHCA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Chemokine signaling pathway, Chronic myeloid leukemia, Dilated cardiomyopathy, ErbB signaling pathway, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis