RTN4IP1 Mouse Monoclonal Antibody [Clone ID: OTI3B2]

CAT#: CF502544

Carrier-free (BSA/glycerol-free) RTN4IP1 mouse monoclonal antibody, clone OTI3B2 (formerly 3B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1. Positive lysates LY409966 (100ug) and LC409966 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502544)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502544)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502544)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502544)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502544)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502544)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502544)

• HEK293T cells transfected with either RC202957 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RTN4IP1 antibody (TA502544), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-RTN4IP1 antibody (TA502544), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-RTN4IP1 antibody (TA502544), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI3B2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RTN4IP1 (NP_116119) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.9 kDa
• Gene Name: Homo sapiens reticulon 4 interacting protein 1 (RTN4IP1), transcript variant 1, mRNA.
• Database Link:
  NP_116119
  Entrez Gene 170728 MouseEntrez Gene 309912 RatEntrez Gene 84816 Human
  UniProt ID Q8WWV3
• Background: This gene encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: NIMP; OPA10

Reference Data

• Protein Families: Druggable Genome