PPM1B Mouse Monoclonal Antibody [Clone ID: OTI3C2]

CAT#: CF502812

Carrier-free (BSA/glycerol-free) PPM1B mouse monoclonal antibody, clone OTI3C2 (formerly 3C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Positive lysates LY419156 (100ug) and LC419156 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC212918 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPM1B antibody (TA502812), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3C2
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPM1B (NP_002697) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.5 kDa
• Gene Name: Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1B (PPM1B), transcript variant 1, mRNA.
• Database Link:
  NP_002697
  Entrez Gene 19043 MouseEntrez Gene 24667 RatEntrez Gene 5495 Human
  UniProt ID O75688
• Synonyms: PP2C-beta; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: MAPK signaling pathway